very well said. i was surprised you ran 33 cycles, we usually go up to 25. and so glad you are explaining how it is to be used as it was designed by Dr. Kary Mullis. as he stated so many times, it is NOT a tool to be used to detect infection. as it has been misused these past 3+ years to do just that... too bad he died August 7, 2019 ... we could have used his voice these past few years.
I did a google search for "confocal microscope image," and saw the image of an embryo stained to show DNA and microtubules that is shown ~50 seconds into this video. I immediately HAD to follow the link, because I made that image when I was in grad school! Funny coincidence.
So a laser beam illuminates specimens pinpoint target on or below surface of the specimen. The target area is scanned rapidly across a few cell thick radius at a time. Different focal planes and depths allow for a 3D to be made on real-time from the signals back from the light collected. The confocal microscope has a high resolution, high magnification, it can also be used for whole specimens which are living without being invasive - it must however be fluorescent which can cause artefacts
Best physics professor!!!!!!! And amazing chem professor at the end that threatened to cut me in half!!!! Richardson's really not good at advertising stuff though...
very well said. i was surprised you ran 33 cycles, we usually go up to 25. and so glad you are explaining how it is to be used as it was designed by Dr. Kary Mullis. as he stated so many times, it is NOT a tool to be used to detect infection. as it has been misused these past 3+ years to do just that... too bad he died August 7, 2019 ... we could have used his voice these past few years.
Gracias por la explicación
Intresting, very nice
Useful.
nice
I did a google search for "confocal microscope image," and saw the image of an embryo stained to show DNA and microtubules that is shown ~50 seconds into this video. I immediately HAD to follow the link, because I made that image when I was in grad school! Funny coincidence.
So a laser beam illuminates specimens pinpoint target on or below surface of the specimen. The target area is scanned rapidly across a few cell thick radius at a time. Different focal planes and depths allow for a 3D to be made on real-time from the signals back from the light collected. The confocal microscope has a high resolution, high magnification, it can also be used for whole specimens which are living without being invasive - it must however be fluorescent which can cause artefacts
Best physics professor!!!!!!! And amazing chem professor at the end that threatened to cut me in half!!!! Richardson's really not good at advertising stuff though...
thank you so much. Perfectly described in just about two minutes!
Very instructive, thank you