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IonOptix LLC
Приєднався 11 тра 2017
Best Practices - Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slices
In this virtual demo, Drs. Adam Veteto and Hanan Qasim show how to successfully acquire force, resting sarcomere length, and calcium data from cardiac slices, including tips and best practices.
Key topics include:
- How to maximize force development in cardiac slices.
- How and why to acquire resting sarcomere length.
- How to best load calcium indicators in cardiac slice and optimizing fluorescence signal-to-noise ratio (SNR).
0:00 Workshop housekeeping
2:42 Introduction
3:22 Dissection and preparation
14:49 Optimizing force development
18:20 Acquiring resting sarcomere length
21:49 Measuring intracellular calcium
29:12 Conclusions and acknowledgements
33:00 Questions and answers
Key topics include:
- How to maximize force development in cardiac slices.
- How and why to acquire resting sarcomere length.
- How to best load calcium indicators in cardiac slice and optimizing fluorescence signal-to-noise ratio (SNR).
0:00 Workshop housekeeping
2:42 Introduction
3:22 Dissection and preparation
14:49 Optimizing force development
18:20 Acquiring resting sarcomere length
21:49 Measuring intracellular calcium
29:12 Conclusions and acknowledgements
33:00 Questions and answers
Переглядів: 26
Відео
Contractility Algorithms for Primary and Cultured Myocytes
Переглядів 271Рік тому
Contractile performance in cardiomyocytes has long served as a key measure of cardiac response to compound treatment as well as genetic modification and pathophysiological conditions. IonOptix offers several contractility algorithms suited to different cardiomyocyte preparations. In this comparison video, we identify the appropriate algorithm to use for primary or cultured cells, as well as spe...
CytoSolver Fitting Paced and Unpaced Cardiomyocytes
Переглядів 56Рік тому
CytoSolver automatically analyzes calcium and contractility traces from both primary cardiomyocytes and iPSC-CMs. It offers a comprehensive set of tools for analyzing fluorescence and motion data collected with IonOptix systems. One of its key features is the ability to fit data from both paced (or stimulated) and unpaced cardiomyocytes. In this video, we describe how to handle data from both. ...
CytoSolver Batch Analysis Software
Переглядів 47Рік тому
CytoSolver automatically analyzes calcium and contractility traces from both primary cardiomyocytes and iPSC-CMs. It offers a comprehensive set of tools for analyzing fluorescence and motion data collected with IonOptix systems. One of the key features of CytoSolver is its ability to conduct automated batch data fitting and analysis with minimal manual intervention. Included with CytoSolver is ...
CytoSolver 3 DataVis
Переглядів 27Рік тому
DataVis allows our users to plot their calcium and contractility data. This video describes how to use DataVis within our CytoSolver 3 platform. If you're a new user or if you just want to see how the software works, check out the video! For more information on our CytoSolver platform, see: www.ionoptix.com/products/sof... or contact us at: www.ionoptix.com/contact/ See more videos and product ...
CytoSolver Batch Analysis Video Tutorial
Переглядів 39Рік тому
CytoSolver 3 simplifies calcium dynamics and contractility data analysis in primary cardiomyocytes, iPSC-CMs and 3D culture models. This advanced tool streamlines data analysis by automating analysis processes, data averaging, and curve fitting, providing researchers with rapid and accurate results. This video describes how to perform analysis using our CytoSolver 3 software. If you're a new us...
IonOptix Cardiac Slice System
Переглядів 271Рік тому
The IonOptix Cardiac Slice System offers fast, real-time, quantitative data acquisition, including calcium and mechanical measurements such as force and work. Find out more: www.ionoptix.com/products/systems/cardiac-slice-system/
CytoMotion Lite
Переглядів 2162 роки тому
CytoMotion Lite system is a low-cost solution offering fast, real-time characterization of contractility kinetics from stem cell-derived cardiomyocytes. Find out more: www.ionoptix.com/products/systems/cytomotion-lite-system/
iPSC-derived Cardiomyocyte Contractility Acquisition and Analysis
Переглядів 7013 роки тому
Interested in characterizing iPSC-CM contractility? This recording from our live demonstration shows contractility measurements of spontaneously beating iPSC-derived cardiomyocytes pre- and post-isoproterenol treatment using our CytoMotion software and MultiCell system. After acquisition, we show how to analyze and visualize data using our CytoSolver analysis. Contents: 0:00:00 - Introduction 0...
Cantilever Replacement tutorial
Переглядів 533 роки тому
The IonOptix MEMS force probe is the next generation force sensor for our optical fiber interferometry-based force transducer designed to detect the nanoscopic forces from single isolated cardiac cells. The implementation of a high performance optical fiber reduces the size of the device, extending the experimental area while protecting the fiber. The MEMS force probe features a modular design ...
CytoMotion: Fast, simple, reliable, real-time measurements of iPSC-CM function
Переглядів 4603 роки тому
Acquiring contractility kinetics in human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) derived cardiomyocytes can be especially challenging given their amorphous phenotype and poorer contrast relative to adult ventricular cardiomyocytes. We've made it simple. Our CytoMotion software enables analysis of the following critical parameters: Max contraction velocity...
Understanding Cardiovascular Physiology at a Mechanistic Level
Переглядів 1913 роки тому
How do you understand cardiovascular physiology at a mechanistic level? Fast, real-time, high-content calcium and contractility data with precise batch analysis. That's IonOptix MultiCell. www.ionoptix.com/products/systems/multicell-high-throughput-system/
MultiCell Lite Highlights
Переглядів 1773 роки тому
Faster data collection with easier to use, streamlined acquisition and analysis. MultiCell Lite makes characterization of excitation-contraction coupling in isolated cardiomyocytes more powerful than ever. Available as a standalone system or as an upgrade to existing IonOptix Calcium and Contractility Systems. Contact us for more info: www.ionoptix.com/products/systems/multicell-high-throughput...
IonOptix C-Pace EM Culture Stimulator Tutorial
Переглядів 9863 роки тому
IonOptix Culture Pacers have been on the market for nearly 20 years and have been used in hundreds of peer-reviewed publications. This tutorial walks viewers through system basics, including how to set up and use various pacing modes as well as electrode cleaning procedures. For more system information, please visit the product page at: www.ionoptix.com/products/systems/cell-culture-pacing/ or ...
Murine Cardiomyocyte Isolation
Переглядів 4064 роки тому
Isolated cardiomyocytes represent the smallest fully functional model system of heart muscle and are of particular importance to the study of cardiovascular disease. In this video tutorial, we describe some of the trickier aspects of a typical Langendorff-based murine cardiomyocyte isolation, including stereoscopic video footage of the aortic cannulation as well as what to look for when digesti...
Hi. I'm trying to use it in skeletal muscle fibers. Do you know if it is possible? Thanks.
Hi Debora, we've used our sarcomere length algorithm to measure contractility in skeletal fibers. It's a little trickier than cardiomyocytes because they tend to twist when isolated, but it's definitely do-able and has been published many times in the past. Contact us at www.ionoptix.com/contact/ or email us (info@ionoptix.com) if you'd like more information.
What do you see if you plot pixel intensity against pixel correlation? Is one preferable to the other for quantification of kinetics?
Thank you for that tutorial. That makes me more prone to understand the underlying efforts of the pyhsiological studies about AF pathogenesis.