Відео

Thank you very much for your kind feedback.

Hello. Once you follow the instructions given in this video, you will get a number of measurements. Find the minimum and maximum values and you can express those values as a range (min-max nm).

If in the SEM picture the unit is a micrometer then selecting the scale which unit we have to put tmicrometer or nm? I need a measurement of nanoparticles in nm.

@@azmatullah4555 You have to use the same units. Otherwise, it will not fit the image scale.

Thank you so much ​Okay it means that if SEM image has 10 micrometer then I have to put unit micrometer in selecting scale @@amc-tec7721

@@azmatullah4555 Yes. That's correct.

The Min and Max values represent the grayscale value ranging from 0 to 243. This gives an insight into the Z-axis variation of the profile (minimum depth and maximum height). If a particular pixel is lighter, it means a higher Z-axis value.

The problem solved, I have saved it in PNG form

​@@aminadz7890 Happy to know that you fixed the issue. Also, check ua-cam.com/video/4xAKFYFABDI/v-deo.html You will find that "Overlay" is unchecked. This is important.

Thank you very much for your feedback.

It's ImageJ. An opensource sofrware.

Could you please ask the question in English?

You can contact me via email (isurul@sci.pdn.ac.lk). Also, you can post your question here, and I'll reply to it.

Feel free to ask your questions.

Hello. Thank you for your comment. What specifically do you want to know about these graphite crucibles? Feel free to ask for any details.

@@amc-tec7721 Hi, mostly just looking for the dimensions of the crucible, or if it's not too much trouble could you provide a link from where I could obtain one? That would also be extremely helpful. Thanks!

@@Malzzel Hello, These are the crucibles that we ordered. www.aliexpress.com/item/1005001547841703.html?spm=a2g0o.order_list.order_list_main.36.42351802C9GWhN

@@amc-tec7721 Great, thank you so much! :)

Since we input "nm" as the unit, it will give all the dimensions in "nm".

@@amc-tec7721 ok thnak you 😇

I'm really happy since this was helpful for you.

This is from the MTI group, USA. www.mtixtl.com/index.aspx

Thank you for your comment. I think it's better to use BET analysis if you are using porous materials. Even though SEM can provide Z-Axis data using the greyscale, you can not accurately calibrate it. Without the Z-axis, you can't discuss volumes. You can measure pore diameters (and areas) using this method. If you want to get Z-axis details and obtain volume information, use Gwyddion, and you may get some values. I'm not sure about its accuracy.

Sorry for the trouble. I'll take this as an advise for future videos.

Thank you so much for your comment, and happy to hear it helps you.

Sure. I'll make some videos regarding those characterization and related softwares. Thank you for the suggestion.

Sure. I'll make some videos. Until that if you have any doubts about XRD and EDS analysis, don't hesitate to mail me. (aaisurulakmal@gmail.com).

Please check comments below. I have given an answer for this question earlier to another user. Feel free to reply if that doesn't helps you.

Sure. What is it?

Thank you...

Thank you...

After adding custom scale and other texts, try saving it in different file formats (JPG, PNG,...).

9:25, you can see the overlay is unchecked. This is important.

For any measurements using ImageJ, you need a known scale or known length. Without that you can't measure any. In your case, if it's possible to get known distance by taking screenshot, you can go for that and do the measurement. For your publications, you can use the original one. I hope I got your question correctly.

Really appreciate your feedback.

Even though we have placed a new scale, dimension vice, it's the same. So it will not affect the measurements. You can do the same measurement using the customized scale anytime, as done in the first step.

@@amc-tec7721 Does as done in the first step mean we measure the customized scale bar first. Then, proceed to enter the known distance in the set scale with the value from the scale bar. After that, do measurement for the sample. Am i understand it correctly?

@@nurmaaeina2083 Yes. You are correct.

@@amc-tec7721 Alright, thank you Sir.

You must need to have any known distance in the image. As an example, if your sem image contains a micro-scale circuit and if you know the distance between two current paths, you can take that as a scale bar. If the image is the original one from the SEM machine (.TIF file format), you can use Gwyddion software without having a scale bar.

The important thing is visibility. You may add several SEM images together in your manuscripts. In this case, you have to reduce the size of the images so they may not visible clearly. In this case, it's better to extend the scale a bit. This is a good practice for manuscript writing.

@@amc-tec7721 which means, the 500nm label is an example not the true size of the SEM image printed?

@@nurulnawwaraliaabintimahmo778 It is a true scale. Look carefully at what happened to the scale when I change the value to 500 nm. It relatively increases. I hope you understand the basic fundamentals of a scale. It's a relative one. Even though you change the scale to any value you like, the measurements taken using that scale must be the same. You can try different scales and measure a specific particle or grain or any object in the images. It should give you the same measurement. Feel free to ask if you have any doubt.

@@nurulnawwaraliaabintimahmo778 Also, it is not compulsory to put the exact scale printed in the original SEM image.

ImageJ

To view, you can use any image viewer including windows image viewer. For the analysis purpose, I used ImageJ software.

Of course, you can change the scale to any clearly visible value. Any journal accepts that.

Please use this link to download the software. imagej.nih.gov/ij/download.html

Consider you have a distribution of data and you need to plot a histogram. Simply, partitioning the data in the distribution to the number of groups in an ascending or descending order can be named as binning and one of that groups is a bin.

I'm really happy if this helpful for you.

Sure. What do you have to measure? If you want, contact me through the mail. (aaisurulakmal@gmail.com)

Dear Mahesh, It's my pleasure to help with this regard. If you need to calculate trichome density, you have to select a known area and count the number of trichomes. In this case, it's better to select your lower magnification images so you can count higher numbers. It will reduce the standard deviation. For that, first, you have to set the scale as I described in the video. In the top left of the image, you can find the dimensions of the image. So you can find the area of the image. Once you count the number of trichomes, by dividing it by area, you can find the density of trichomes. If it is too difficult to count in a large area, you can crop the image and get a smaller area. Since the trichomes are not linear, you can't measure the length with the straight line tool. In your case, you have to use segmented line tool. You can access it by right clicking on the straight line tool. In my video I have used two points to draw a straight line and in your case, you have to put several points to measure curved objects. When you put this points on Image, you can put the last point by right clicking. Finally you can measure the lengths as described in the video. Good luck.

@@amc-tec7721 Thank you sir

ImageJ can be used to analyse images. So you have to get two images in a known time frame. In both two images, mark a reference point below the bubble (consider a fixpoint of the images such as a scratch of the solution container). Measure the distance between the bubble and the reference in two images and subtract them so you know the distance it travelled. Since you know the time it took, you can find the velocity.