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Wellcome Centre for Anti-Infectives Research
Приєднався 17 сер 2020
CHI LogD Assay
Lipophilicity is a key parameter which governs the pharmacokinetic properties of a drug. CHI LogD is a reverse phase HPLC method used to measure the percent organic phase concentration by which a compound is eluting. This has a direct relationship to lipophilicity and can be used to indicate the effect on a compounds pharmacokinetic properties.
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Відео
Suspension Hepatocyte Assay Training Video
Переглядів 356 місяців тому
Suspension Hepatocyte Assay Training Video
hERG channel blockers: Part 2
Переглядів 176Рік тому
This video takes you through how to chemically avoid blocking the hERG channel. Many drugs can find this a stumbling block in their drug discovery process, so it is important to know how to design around this. It is advised you watch Part 1 before watching this video. #drugdiscovery #education #heart #science #subscribe @wellcomecentreforanti-infe6171
hERG blockers and how to avoid making them. Part 1
Переглядів 412Рік тому
Why do drug discovery scientist care so much about the hERG channel. In this short talk learn what the hERG channel is and it physiological function, why blocking this channel is not desirable and what kinds of drugs bind to this channel. There are some problem solving questions in there too. Enjoy! Why not subscribe to our channel for more videos on drug discovery @wellcomecentreforanti-infe61...
Bioanalysis for in-vitro assays
Переглядів 132Рік тому
How and why do we prepare bioanalytical samples that have come from in vitro experiments? In this video, we will take you through both the microsomal clearance and MDCK permeability experiments. #DMPK #massspectrometry #education #science
How to perform the MDCK Permeability experiment in drug discovery
Переглядів 773Рік тому
In this interactive video, we take you through the step by step process on how to set-up and carry out the cell permeability assay. Included in this video is how to seed your cells and analyse the permeability results at the end. 0:00 Introduction 03:10 Equipment and consumables 08:15 Day 1 Seeding 12:59 Day 5 Assaying 23:54 Data Analysis 25:40 Advantages and disadvantages check out more videos...
How to set-up a heated reaction using an oil bath, sand bath and a heating block
Переглядів 2 тис.Рік тому
In this short video we will walk you through how to set-up a heated reaction using an oil bath, a sand bath and a heating block. PPE must be worn at all times when carrying out any chemical reaction. 00:00 Introduction 00:33 Using an oil bath 02:47 Using a sand bath 03:23 Using a heating block Check out more videos by @wellcomecentreforanti-infe6171
How to set-up a hydrogenation reaction using palladium on carbon and a hydrogen balloon
Переглядів 9 тис.Рік тому
In this short video we will explore how to set-up a hydrogenation reaction using palladium on carbon and a hydrogen balloon. Care and attention is required when handling hydrogen gas due to its explosive nature. PPE must be worn when carrying out any chemical reaction. MSD files must be read prior to carrying out any chemical reaction. 0:00 Introduction 01:02 Preparing the Hydrogen Balloon 02:4...
Making a Grignard reagent from elemental magnesium
Переглядів 1,2 тис.Рік тому
In this short video, we will show you how to carry out making a Grignard reagent from elemental magnesium. Additionally, you will be shown how to use a canula to isolate the newly formed Grignard reagent. PPE must be worn when carrying out any chemical reaction. Caution must be taken when handling reagents and chemicals. Always refer to the MSD files for every reagent. An ice bath must always b...
How to set-up anhydrous chemical reactions using a vacuum line and a nitrogen balloon
Переглядів 607Рік тому
This short video will take you through how to set-up an anhydrous reaction using a nitrogen balloon as the inert atmosphere. A vacuum line is used to purge the system from air. PPE must be worn at all times when carrying out scientific lab work. Check out more videos by @wellcomecentreforanti-infe6171
Mass Spectrometry: Electrospray
Переглядів 704Рік тому
Electrospray is the most commonly used source of ionisation in both medicinal chemistry and DMPK. Join Paul Scullion to learn about what is electrospray ionisation and how liquid chromatography conditions affects the response of the analyte.
Which Mass Analyser should I buy and why?
Переглядів 156Рік тому
How do you choose which mass spectrometry instrument to buy? What information do you need from the machine? This short video highlights the strengths and weaknesses of the machines to assist you to pick the best.
In Conversation With... Andrew and Cedric. Medicines Without Frontiers?
Переглядів 252 роки тому
Host: Lauren Webster. Speakers: Andrew Mtewa, Cedric Graebin Producers: Ali Floyd, Ailsa Mackintosh, Suze Farrel, and Catharine Goddard Follow us on Twitter and Instagram: @WCAIRDundee www.wcair.dundee.ac.uk
Introduction to Pharmacokinetics Part 2
Переглядів 2932 роки тому
This second talk continues to describe what the body does to the drug as a function of time. It incorporates ADME/T
Mass Spectrometry: Mass resolution and elemental composition
Переглядів 3592 роки тому
Mass Spectrometry: Mass resolution and elemental composition
In Conversation With Kevin, Luke, and Ngaire
Переглядів 412 роки тому
In Conversation With Kevin, Luke, and Ngaire
How to set up dreschel bottles to scrub chemical reactions
Переглядів 2,1 тис.2 роки тому
How to set up dreschel bottles to scrub chemical reactions
How to safely clean up a liquid chemical spill in a fume hood
Переглядів 4712 роки тому
How to safely clean up a liquid chemical spill in a fume hood
How to handle a solid chemical spill in a fume hood
Переглядів 1942 роки тому
How to handle a solid chemical spill in a fume hood
WCAIR NMR electronic and magnetic effects
Переглядів 602 роки тому
WCAIR NMR electronic and magnetic effects
Thanks fpr the detailed explanations :)
thanks
I’m watching you adding ethanol and the predissolved reactant and I have already two questions: how can be the ethanol dry and the reactant moisture/water or oxygen free if you’re adding it this way?
NEVER use septa for hydrogenations. They will leak when pierced. That leak could blow atmospheric gases over your catalyst in a flammable solvent when you do the last purge. Then your flask can spontaneously disassemble with a ear drum destroying conclusive blast. You will kill your lab partner and wished you paid attention to this comment because now I'll tell you to ALWAYS use a 3 way vacuum adapter with the standard tapered glass joints. P.S. I've never been in a chemistry class. Not in highschool and definitely not in college. Even I know these things. That means there is no excuse for ignorance that leads to catastrophic events.
Which oil is used?
Very nice video, two comments; once punctured, septa are not terribly vacuum-tight, meaning under vacuum, atmospheric air could be sucked in through the puncture. If the volume of hydrogen is completely consumed by the reaction, the setup could generate a partial vacuum, which once again could lead to atmosphere ingress.
Great video!
Thanks WCAIR; I appreciate this video!
Hey, I was just wondering if you have to evacuate the air out of the flask and back-fill it with nitrogen if I were using a nickel catalyst? Does it effect the hydrogenation reaction or is it evacuated just because it reacts with the palladium? I'm doing a school lab investigation on finding the activation energy of hydrogenation, so I was just wondering if that step would be necessary for my experiment. Thanks!!
I have a question, how much would a setup/lab cost if you would like to do hydrogenation "industrially"? It's because I have an idea for a product I'd like to create, synthesize like this and I wondered how much this would cost on average & the amount of substance one could "hydrogenate" at a time? Thanks
Is the “product” a substituted amphetamine? 😐😐😐
Or phenethylamine? That alpha methyl doesn't make all the fun 😅
Thanks
Great explanation
I like how you injected the hydrogen with the balloons and the syringe but shouldn't the space be flooded, I assume you just have to wait a lil longer for the correct amount of gas or equalization, just never seen it done like that, it was a good idea
Hi there. not sure what you mean by "the space be flooded"? Let me know how we can help you with that bit.
@@wellcomecentreforanti-infe6171 you are vacating the air from the flask with hydrogen, making it a hydrogen rich enviroment, never seen someone do it with a syringe before, my comment was pretty rudimental
@@shawnio Great!
It is said that oxytocin can make people loyal. Have you ever extracted oxytocin?@@wellcomecentreforanti-infe6171
@@wellcomecentreforanti-infe6171It is said that oxytocin can make people loyal. Have you ever extracted oxytocin?
Excellent work that deserves admiration ..... I love the miniature industrial processes that take place in the laboratory ..... Excellent work .. Excellent work ... Thank you very much
Thankyu
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@@wellcomecentreforanti-infe6171Brother, it is said that oxytocin can make people loyal to love. Have you ever extracted oxytocin? It can also make people more loving.
If the ether is not sodium dried, the reaction yield will be very low.
Clear explanation hats off to your efforts dir
O' HOW I DISLIKE LOONIE MURDERING AMERIKINS
░p░r░o░m░o░s░m░ 🎶
Fantastic effort at demystifying AI/ML.. As usual, appreciate your ways of using simple tools and language to make the point...
Very much enjoyed the story narration. Great to see you on the video screen again.
Superb kaushik sir😊very impressive work.Story and story telling is very professionally done. I enjoyed every moment of it👏🏻👏🏻👏🏻
Love the ending😅
I never got why Physics is considered a science. It feels more like a math.
Nice, 👍 all the way from Texas to here, are you used to our climate yet? lol wet and cold!!!
Mattie insists that she loves it in Dundee - it is the sunniest city in Scotland, after all*
*not a guarantee ;)
Really interesting. Thank you for sharing your story. CH.