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Dr. PREM-PRIMER Biotech Lectures
India
Приєднався 9 жов 2021
Since I am a researcher turned teacher, I use self-explanatory cartoons and animations to educate students about rDNA technology. I hope my presentations will serve you as a primer for your learning.
Відео
Difference between Apoptosis and Necrosis Part-2
Переглядів 547 місяців тому
Difference between Apoptosis and Necrosis Part-2
Differences between Apoptosis and Necrosis part-1
Переглядів 977 місяців тому
Differences between Apoptosis and Necrosis part-1
Apoptosis: Defination, Morphological and biochemical features
Переглядів 1648 місяців тому
Apoptosis: Defination, Morphological and biochemical features
Barren: Infertile, soil not good enough for plants to grow.
Переглядів 229 місяців тому
Barren: Infertile, soil not good enough for plants to grow.
cDNA Library: Constructions, Types and Vectors, Applications
Переглядів 2959 місяців тому
cDNA Library: Constructions, Types and Vectors, Applications
Corollary: Natural Consequence of Something
Переглядів 159 місяців тому
Corollary: Natural Consequence of Something
Cantankerous: Bad tempered, always complaining
Переглядів 429 місяців тому
Cantankerous: Bad tempered, always complaining
cDNA Synthesis from RNA
Переглядів 2,7 тис.Рік тому
The conversion of eukaryotic mRNA into DNA using a Reverse Transcriptase and the DNA generated in that process are complementary to the initial mRNA; hence, that DNA is termed as cDNA. Reverse transcriptase catalyse the in vitro synthesis of DNA by using mRNA as a template process is called Reverse Transcription. Generally, the cDNA is prepared in the laboratory which is different from the Euka...
What is Genomic Library: Definition and Construction
Переглядів 333Рік тому
Genomic Library: A set of recombinants clones that contain all the DNA present in an individual organism Genomic Library construction has five majors steps 1.ISOLATION OF DNA 2. FRAGMENTATION OF DNA 3. LIGATION OF VECTOR AND DNA FRAGMENTS 4. INTRODUCTION INTO HOST CELL 5. SCREENING & SELECTION OF RECOMBINANTS
Homopolymer tailing: DNA Joining Molecules
Переглядів 1,2 тис.Рік тому
Homopolymer tailing: DNA Joining Molecules
Linkers: Generation of Cohesive ends on DNA fragments
Переглядів 48Рік тому
Linkers: Generation of Cohesive ends on DNA fragments
Differences Between Linkers and Adopters, Cloning of blunt End DNA fragments
Переглядів 59Рік тому
Differences Between Linkers and Adopters, Cloning of blunt End DNA fragments
Adaptors: Joining Molecules, Blunt END DNA fragments cloning
Переглядів 166Рік тому
Adaptors: Joining Molecules, Blunt END DNA fragments cloning
Linkers: Joining molecules, Cloning of Blunt DNA Fragments/ cDNA molecules
Переглядів 207Рік тому
Linkers: Joining molecules, Cloning of Blunt DNA Fragments/ cDNA molecules
JAWAHARLAL NEHRU SCHOLARSHIPS FOR DOCTORAL STUDIES IN SOCIAL SCIENCE
Переглядів 55Рік тому
JAWAHARLAL NEHRU SCHOLARSHIPS FOR DOCTORAL STUDIES IN SOCIAL SCIENCE
M13mp7 Cloning Vector: Symmetrical restriction sites
Переглядів 129Рік тому
M13mp7 Cloning Vector: Symmetrical restriction sites
Palindromes: Restriction sites, Restriction Endonucleases
Переглядів 92Рік тому
Palindromes: Restriction sites, Restriction Endonucleases
Construction of M13mp2: M13 Cloning vectors
Переглядів 407Рік тому
Construction of M13mp2: M13 Cloning vectors
Cohesive ends Blunt ends: Restriction endonucleases
Переглядів 345Рік тому
Cohesive ends Blunt ends: Restriction endonucleases
linear DNA vs Circular DNA Restriction digestion
Переглядів 322Рік тому
linear DNA vs Circular DNA Restriction digestion
thank you very much keep going please
@@YALEMQUEENS-nn2xu Welcome and happy learning
Cen 6 equal distribution
Why don't you talk in hindi?
@@Biology60___ hi, it won't be very effective in himdi
Well done, Sir! Thank you so much. This is really helpful.
Thank you & welcome. Try to subscribe to the channel for more updates
excellent class sir, thank you .
You are welcome, try to subscribe channel for more updates
Thank you for this video! Helped me a lot ! If I want to add a fusion protein so that I can increase the solubility of my protein, is the fusion protein inserted into the vector along with my gene? I got lost in the order of the plasmid construction steps..
Hi, Thanks for the nice comment You choose the Expression Vector with various tags (For example GST tag) to increase the solubility and stability of your protein. I suggest expression vector with GST tag may serve your purpose.
@@Dr.DNA-Primer thank you very much !
👍🏻
All the best
Can we use the baculovirus expression system to express the avian viral proteins?
Any gene can be expressed in baculovirus expression irrespective of the origin of gene. Try to subscribe channel to get regular updates.
Thank you sir
So nice of you
Very helpful 👍🏽
Thank you, don't miss updates of the channel try to subscribe. 😀
Thank you
Welcome🎉, do subscribe channel for updates
perfect
Thanks do subscribe for more updates
Thank you and do subscribe for more updates from channel
Great video
Thanks and try to subscribe for updates of channel
👍🏻👍🏻
🙏🙏🙏
Great explanation! Thank you.
Thank you & welcome
Good lecture. Also pl give a talk on engineered M13 phage for peptide synthesis
Thank you, soon I will give talk on that topic
thank you Dr.k for your great explanation
Welcome, try to consider my channel for subscription and will get you updates
👌 "promosm"
❤
Thank you sir 😊
Welcome
👍
🙏🙏🙏
Good presentation sir
Thank you 🙏 Sams Chemistry
It's an Amazing Explanation Sir, Thanks for the Video🙏🤝
Thank you and do subscribe for updates
@@Dr.DNA-PrimerSubscribed Sir, Thank you😇
All the best
Wow this video is very helpful sir... I hope you see my comment Your explanation is very good and crystal clear...your students are lucky to have you ❤
Thank you
Such as a wonderful explanation sir thank you ❤🎉😊
Welcome
Sir, what is single strain system in packaging of lambda cloning vector?
Mutation in Cos sites of Lamda DNA can not undergo replication but can synthesize all phage proteins. Such proteins are purified and mixed with recombinant lamda molecule and then phage particles are formed in vitro. Mutations in Cos sites lambda DNA phage strain is sufficient to pack the recombinant lamda DNA.
Tdt does not require template strand so it is called template independent polymerase ... sir does this involve in exonuclease activity
One of the variant of Mammalian TDT have 3'to 5' exonuclease activity
Thank you sir😊
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Sir I have seen your two videos on reverse transcriptase.. But I have a doubt reverse transcriptase enzyme need template OR not for synthesis of cDNA. If need template than which template
Hi, During the retroviral infection, by using Reverse transcriptase RNA is converted into double standard DNA and that is integrated into host genome. Eukaryotic mRNA will be used as template to synthesize cDNA by employing Reverse transcriptase.
@@Dr.DNA-Primersir reverse transcriptase requires a primer, which in the case of retroviruses, is a tRNA molecule bound at a specific site (the primer-binding site) close to the 5' terminus of the viral RNA
Yes
Good one..
Thank you
Sir I have a question, while making phagemid vector do we insert ss DNA fragment of M13 into the plasmid
No, double standard M13 fragment will be inserted. M13 genome converted into double standard that is called replicative form. Those replicative forms can be isolated.
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Thanks a lot sir 🙏 I was really confused about this
You are most welcome,
Sir, how primers are prepared chemically or using primase enzyme
Chemical Synthesis only
During in vivo replication primers are synthesized by primases only
For pcr and RT-Pcr chemically synthesized primers are used
@@Dr.DNA-Primer thank you sir
Did you subscribe me
18:43 sir u said that wrong nucleotide are added how they are corrected is their any proof reading activity
Generally Reverse transcriptase do not have 3' to 5' exonuclease activity (Proof reading activity) so no correction of wrong nucleotides added. Thanks
@@Dr.DNA-Primer thank you sir
Super sir
Thank you
Good explanation
Thank you
Thank you for explaining it so well.
Welcome and try to subscribe for updates
Nice presentation sir
Thank you
Liked and subscribed.too..it is very useful prem.
Thank you Sneha
Thank you so much sir for this lecture. ❣️
Welcome, Please share our videos with needy friends
Thank you Sir 😊
Most welcome
Sir i applied but i didn't get any email id
you did not get any mail from the Reliance foundation, wait few days or write to them
Can you please make a vedio of primer designing for PCR,RTPCR and sequencing. It will be helpful for many students
I will do certainly, I may take couple of weeks to do
The same fellowship is renewed after 6 month? becoz my father retire after three months then his income changed to less than 8 LPA.
Hello there, The applicant shall be the first year student of M.Sc/ Ph.D in lifesciences. After six months you may be in second year of M.Sc or Ph.D then you will not eligible. I am guessing for every one year there will be a notification from Bayer. Hope I answered to your question.
Sir i am very depressed i enroll phd in botany work on plant patho 1st year from pune university but i am in open category my father is teacher but my father dont me to do phd becouse as of now phd is expensive for them sir plz suggest me any other fellowship for open category.i need support if gov dont give me any fellowship may be i am give up next year.
Hi, please look for a part time teaching job and continue your Ph.D Programme. You can write the UGC-CSIR exam in the meantime. If you qualify for that and you will get fellowship for five years,
Thank you for the effective information sir✨ and I am having two doubts 1) fellowship is like scholarship or we have to work for them like internship because in that it's mentioned as industry exposure. 2) interview will be online or we have to go to any other states and attend it? So, pls clarify my these two doubts which would be very useful for me.
Hello Abirami, thanks for complement. Regarding your first question fellowship is monthly stipend and you need not work for them. By using that you will complete masters or Ph.D programme. I am not sure about interview mode offline or online. I am guessing it would be online. All the best. Dont forget to 😄subscribe my channel for many updates
@@Dr.DNA-Primer Okay sir.. Thank you so much ✨👍
Sir Mera Master's cmplt last year in Zoology also clr ICAR Ph.d entrance exam in Biochemistry So can I apply this form or n't in Ph.d ??
If you have joined in Ph.D Program, then you can apply for this fellowship under Ph.D category.
Sir I recently completed my pre PhD course and not getting resistration no. From the University and also getting University fellowship 8000 per month di I apply for this fellowship
Hello there, if you are in first year of Ph.D program and not receiving fellowship then you are eligible to apply. during the application you need produce a document from the university stating that you are not availing any fellowship.
Sir, Ph.D in Agricultural Engineering students can apply this fellowship
Its for Ph.d in lifesciences, So you can try
Sir send your mob. No.
you can write to me dnadeeds@gmail.com
👍
thank you
Thank you for explaining in a simple manner
Thank you