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Rohini Patil
Приєднався 10 вер 2015
Western Blotting
Western Blotting is a technique to screen the cDNA library. It allows selection on the basis of expressed protein detection.
Переглядів: 15
Відео
Dot and Slot Blot
Переглядів 193 місяці тому
Dot blot and slot blot are technique to identify recombinant. It does not involve electrophoresis.
Colony Hybridization and Plaque Lift Assay
Переглядів 464 місяці тому
Colony hybridization and plaque lift assay are screening methods. The methods involve in situ hybridization. You may contact patilrohini194@gmail.com
Northern Blotting/ Hybridization
Переглядів 724 місяці тому
The method used to detect the RNA by blotting technique is known as Northern blot.
Blotting Techniques
Переглядів 244 місяці тому
Blotting is transfer of nucleic acid to nylon or nitrocellulose paper. The technique is utilized in southern , northern, western etc. blotting/ hybridization.
Southernwestern Blotting
Переглядів 364 місяці тому
Southernwestern Blotting technique is for identification of DNA-protein interaction.
Optimization of Codon/Codon Choice,mRNA structure:Factor Affecting cloned gene Expression in E. coli
Переглядів 302Рік тому
The codon optimization is important for high level expression of foreign gene with accuracy. At the same time mRNA structure is also affecting expression of gene is discussed. You may go through Plasmid stability and Plasmid copy number ua-cam.com/video/-NsQqalAwwU/v-deo.html Optimization of translational initiation sequence ua-cam.com/video/hZssThZCG3M/v-deo.html Transcriptional termination ch...
Plasmid stability and plasmid copy number:Structural and segregative stability,incompatibility
Переглядів 404Рік тому
Plasmid copy number of vector affects expression of gene. The vector with high stability has to be selected for stable gene expression of foreign gene in E.coli host. You may go through: Promoter strength ua-cam.com/video/I_MC4fizMEY/v-deo.html E.coli expression vector:cloned gene under controllable promoter ua-cam.com/video/13DGQN3yHEk/v-deo.html Optimization of translation initiation sequence...
Optimization of Translational initiation sequences 5՚UTR for cloned gene in E.coli
Переглядів 107Рік тому
Translational initiation sequences affect rate of translation. The SD sequence and other factors are discussed in detail. Transcriptional Termination choice for Expression Vector: ua-cam.com/video/hFf-L8VLEhU/v-deo.html Promoter Strength: ua-cam.com/video/I_MC4fizMEY/v-deo.html E. coli expression vector: Cloned gene under control of regulated promoter: ua-cam.com/video/13DGQN3yHEk/v-deo.html Yo...
Transcriptional Termination Choice for expression Vectors in E.coli
Переглядів 102Рік тому
One of factor affecting expression of foreign gene E.coli is transcriptional termination site. You may contact rohini.coaching@gmail.com
Promoter Strength:lacUV5,hybrid promoters:tac,tic:factors affecting cloned gene expression in E.coli
Переглядів 619Рік тому
For efficient expression of foreign genes in E.coli host promoter is required. in this video the promoters for expression of genes are discussed. Watch more about regulated promoters of E.coli expression vector ua-cam.com/video/13DGQN3yHEk/v-deo.html You may contact rohini.coaching@gmail.com
Response element :Eukaryotic Gene Expression Regulation
Переглядів 254Рік тому
Response elements are cis acting element. Response element are activator binding site. You may contact rohini.coaching@gmail.com
Difference between pUC18 and pUC19/Compare and Contrast:pUC18 and pUC19/high copy no. plasmid vector
Переглядів 956Рік тому
The discussion is regarding similarities and dissimilarities between pUC18 and pUC19 vectors. In detail focuses on characteristics of pUC18 and pUC19 vectors. You may contact rohini.coaching@gmail.com
Alpha Complementation, Blue White selection, pUC18
Переглядів 244Рік тому
Alpha complementation can be used as selection or screening technique. It can be used to select recombinant. You my contact rohini.coaching@gmail.com
Two Hybrid System, Two Hybrid Assay,Yeast Hybrid System,Method to detect protein protein interaction
Переглядів 122Рік тому
Two hybrid assay or two hybrid system is used to detect protein protein interaction. The video explains in detail how is assay carried out. You may contact rohini.coaching@gmail.com
DNA Binding Domain, Activating Region: Zinc Finger,Leucine Zipper,Helix Loop Helix,Helix Turn Helix
Переглядів 910Рік тому
DNA Binding Domain, Activating Region: Zinc Finger,Leucine Zipper,Helix Loop Helix,Helix Turn Helix
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
Переглядів 1,9 тис.Рік тому
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
Global Regulators/Global control system/Global control Network/Global Regulatory System
Переглядів 3732 роки тому
Global Regulators/Global control system/Global control Network/Global Regulatory System
gal operon: Structure, Regulation, two levels of regulation, GalR
Переглядів 2,2 тис.2 роки тому
gal operon: Structure, Regulation, two levels of regulation, GalR
Isolation of Bacterial Genomic DNA: Steps for isolation, methods to purify and concentrate DNA
Переглядів 1872 роки тому
Isolation of Bacterial Genomic DNA: Steps for isolation, methods to purify and concentrate DNA
Finding Concentration of DNA and Purity Check of Isolated DNA
Переглядів 2322 роки тому
Finding Concentration of DNA and Purity Check of Isolated DNA
Plasmid DNA Isolation Methods:Alkaline lysis, EtBr-CsCl density gradient, Isolation on basis of Size
Переглядів 1 тис.2 роки тому
Plasmid DNA Isolation Methods:Alkaline lysis, EtBr-CsCl density gradient, Isolation on basis of Size
Real time PCR/ Quantitative PCR/ RT-PCR/ qPCR
Переглядів 562 роки тому
Real time PCR/ Quantitative PCR/ RT-PCR/ qPCR
Inverse PCR : Variation of PCR / Types of PCR
Переглядів 2172 роки тому
Inverse PCR : Variation of PCR / Types of PCR
Polymerase Chain Reaction/PCR Basic Principle/ Essential Components of PCR/Parameters Affecting PCR
Переглядів 1432 роки тому
Polymerase Chain Reaction/PCR Basic Principle/ Essential Components of PCR/Parameters Affecting PCR
Genetic / Genomic Imprinting: Importance in Epigenetics, When and How imprinting happens: Disorders
Переглядів 5082 роки тому
Genetic / Genomic Imprinting: Importance in Epigenetics, When and How imprinting happens: Disorders
No Go Decay pathway: Dom34/Hbs1, Pelota/Hbs1, Pelota in Begomovirus resistance in Tomato, ty5 locus
Переглядів 2392 роки тому
No Go Decay pathway: Dom34/Hbs1, Pelota/Hbs1, Pelota in Begomovirus resistance in Tomato, ty5 locus
Chromatin remodeling complexes/Antirepressor, HO gene & PHO5 gene Activation
Переглядів 5312 роки тому
Chromatin remodeling complexes/Antirepressor, HO gene & PHO5 gene Activation
Histone Modification: Acetylation, Deacetylation, Methylation Demethylation of Histones
Переглядів 3982 роки тому
Histone Modification: Acetylation, Deacetylation, Methylation Demethylation of Histones
out of so many videos about the same topic this one finally made me clear the concept. Thanks ma'am .
Glad to hear that
plz . i couldnt find the previous lecture about integron .
You will find it in drug resistant play list
So technically the integron integrate gene isn’t really mobile, it’s just a gene cassette that can add and remove ARGs. The real mobile genetic elements here are transposons
Yes
Can you explain what is derepression of gcn4
Depression means removal of repression and to get activated. Now in case of Gcn4, translation of Gcn4 is blocked that is there is repressed condition in high concentration of amino acids. When concentration of amino acid is low the block i.e. repression is removed. It is derepression of Gcn4.
I dont understand how when glactose is being converted to carbon and energy you said the sequence of events was GALACTOSE -> UDP GLUCOSE -> GLU.1.PHOSPHATE-> GLYCOLYSIS When the diagrams show that galactose needs to be converted into GLU.1.PH before UDP-GALACTOSE and then UDP GLUCOSE
Otherwise thank you amazing video really helps me understand !!
Sequence of event is gal to gal-1p to gal-udp to glucose -udp. Glucose -UDP enters glycolysis to give energy.
What about complex transposons
please make a video on serial dilution and bacterial cell counting
Sure
Thank you so much ma'am now I really understand. My lecturer failed to do this❤😢
Thank you
One of the best videos of all time!
Thank you
Thanks a lot 💚your understanding method was easy and sharp
Thank you
If eif 2 is phosphorylated when amino acid is at lower conc , how will it aid other charger trna on the codon . As phosphorylated form of eif2 has higher affinity with gdp and in gdp form its inactive then how will it be continue translation of main orf of gcn4
Remember that not all molecules are phosphorylated at any time.
Kash yehi lecture clg mey karliye hotey too ajj ye nahi dekhney padey hotey 😅
Thank you mam ❤
My pleasure
Thank you mam Lord Buddha bless u
Substantial 🎉TQ mam.
My pleasure
ma'am , Is that etf2 or eif2 ?
eIF2
@@rohinipatil6606 ok ma'am thanks
Nice explanation mam💢
Thank you
Thanks mam
Most welcome 😊
Thank you ma'am for the detailed explanation. I understood all your video lectures.
Thank you
Very recently I came to know about your channel. The videos are very informative. I have a query.......How we can ensure that there is no plasmid contamination during bacterial DNA extraction?
A separate band of plasmid and genomic DNA is visible on agarose gel electrophoresis.
Sorry to bother.....one more query.... What we can do to avoid plasmid extraction during bacterial DNA extraction
Mam please do vedios on computational biology , structural and functional and comparative genomics
Surely
Thank you so much mam🙏🙏
My pleasure
Thank you for the explanation madam...
My pleasure
Very nicely explained Madam. धन्यवाद
Thank you🙏
My favorite teacher of mol bio
🙏🏻
It helped me a lot Thankyu ❤
My pleasure
ThanKs maam ❤
🙏🏻
Plz in Hindi videos
I shall try.
Very helpful Thank you!
My pleasure
I understood it perfectly ☺️ Thank you so much ❤️✨
My pleasure
Very nice explanation, Thank you maam
Thank you
Thank you so much Ma’am for such informative videos which are not available elsewhere. You explained really well. Thank you so much. I would like to know much more about you and request you to share your LinkedIn profile or email id if possible to stay connected.
Thank you. My mail id is given in description box.
Mam please do videos on techniques in rdt
Surely
Cis acting vs upstream
SD sequence is cis acting and it is upstream sequence.
Please make a video on (p)ppgpp metabolism
The video also explains about synthesis and degradation of ppGpp i. e. Metabolism.
DIGE need more explanation..
Ok
I didn't understand how the mismatching increases specificity ??
One of the biggest challenges for microarray-based approaches is to eliminate non-specific hybridization so that probe signals accurately reflect the presence and concentration of specific targets. For that purpose, Perfect match(PM) prob and mismatch(MM) prob are used. Actually Mismatch works as control. To have high accuracy and to avoid false positive result such PM/MM are used. It is specifically for homologous sequences like sequences from gene families. Target is allowed to hybridization with PM and cross hybrisize with MM prob. The signal intensity obtained from MM is subtracted from signal intensity of PM which would eliminate nonspecific signal and reveal actual level of specific hybridization i.e. concentration of real target . In this way mismatch prob help to analyze specificity.
@@rohinipatil6606 Ohk tnq ! ☺️
Ma'am this helped me a lot... Thank you so much ma'am<3
Glad to know. Keep watching.
Hello, great explanation! Thank you. What happens with broken mRNA though? And what is the role of SmpB protein in this process?
Thank you. Good question. RNaseR degrads broken RNA. SmpB interacts with tmRNA and delivers it to stalled ribosomes. C-terminal tail of SmpB is required for accommodation and traspeptidation .
Mam I can't able to understand ,what is different between negative regulation and positive regulation, mam you taught that but get confused will you explain me with example.
To clear your confusion watch video on lac operon, ara operon and trp operon which was uploded.
I can clear my doubts through this lecture..thank you ma'am 🙏
You are always welcome
Thank you from Algeria 🤍
My pleasure
I watching 2 time and now averything is clear.... thank you mam
My pleasure. Keep watching.
Thank you ma'am, it was very helpful!
My pleasure
Thank you so much for posting this!! This was such a clear explanation, helped me understand my classwork so much better <3 much thanks from a first year grad student :)
Very glad to know. Thank you.
Mam can you please make a detailed video on DIGE 🙏
Same video explains DIGE
@@rohinipatil6606 Ok Mam
Very good explanation ma'am!
Thank you
खूप छान....
Thanks for your appreciation🙏
Thank you
My pleasure
Easy to understand ma'am 👍👍
Thank you🙏