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EpiCypher Videos
Приєднався 15 чер 2016
EpiCypher is an epigenetics company founded in 2012 by Drs. Mark Bedford, Or Gozani, Brian Strahl, and James Bone in response to the growing demand for high-quality reagents and tools to study chromatin regulation and enable epigenetics-focused drug development.
EpiCypher continuously pushes technology boundaries to deliver innovative products, solutions, and services to epigenetics and chromatin biology researchers. Most recently, EpiCypher has been at the leading edge of chromatin mapping technology improvements with the recent launch of the highly sensitive epigenomic mapping CUTANA™ assays for ChIC, CUT&RUN, and CUT&Tag.
From our strong scientific expertise and rigor to our focus on customer success, EpiCypher is proud to be an epigenetics company For Scientists, By Scientists.
EpiCypher continuously pushes technology boundaries to deliver innovative products, solutions, and services to epigenetics and chromatin biology researchers. Most recently, EpiCypher has been at the leading edge of chromatin mapping technology improvements with the recent launch of the highly sensitive epigenomic mapping CUTANA™ assays for ChIC, CUT&RUN, and CUT&Tag.
From our strong scientific expertise and rigor to our focus on customer success, EpiCypher is proud to be an epigenetics company For Scientists, By Scientists.
CUTANA™ CUT&RUN Services: Your partner for premium chromatin mapping services
EpiCypher is pioneering the development of CUT&RUN chromatin mapping assays, which provide massive improvements in throughput, data quality, and sensitivity compared to ChIP-based methods. Our services offer exclusive access to EpiCypher’s genomics experts, guaranteeing strong support from experimental design to data analysis.
Learn more about these services and download supporting materials at: www.epicypher.com/services/cutana-cut-and-run
For more information about CUTANA™ CUT&RUN, see:
www.epicypher.com/resources/cut-and-run-overview/
www.epicypher.com/resources/blogcomplete-stepbystep-guide-to-cutrun/
And to get started with CUT&RUN in your lab, check out our comprehensive kits, antibodies, and supporting reagents:
CUTANA™ CUT&RUN Kit: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit
CUTANA™ CUT&RUN Library Prep Kit: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit
CUTANA™ pAG-MNase for CUT&RUN workflows: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows
CUTANA™ CUT&RUN antibodies: www.epicypher.com/antibodies-complete-list/
Learn more about these services and download supporting materials at: www.epicypher.com/services/cutana-cut-and-run
For more information about CUTANA™ CUT&RUN, see:
www.epicypher.com/resources/cut-and-run-overview/
www.epicypher.com/resources/blogcomplete-stepbystep-guide-to-cutrun/
And to get started with CUT&RUN in your lab, check out our comprehensive kits, antibodies, and supporting reagents:
CUTANA™ CUT&RUN Kit: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit
CUTANA™ CUT&RUN Library Prep Kit: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-cut-and-run-library-prep-kit
CUTANA™ pAG-MNase for CUT&RUN workflows: www.epicypher.com/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows
CUTANA™ CUT&RUN antibodies: www.epicypher.com/antibodies-complete-list/
Переглядів: 1 712
Відео
Automated CUT&RUN assays for next-generation chromatin profiling
Переглядів 1,4 тис.7 місяців тому
Biomedical and pharmaceutical researchers are increasingly turning to chromatin mapping assays for identification of new cell types, biomarkers, and drug targets. CUT&RUN is a highly sensitive mapping assay that generates high-resolution profiles for transcription factors, chromatin-associated proteins, and histone post-translational modifications (PTMs) in a fraction of the time and cost of hi...
Chromatin structure, gene regulation, and epigenomic mapping assays
Переглядів 1,4 тис.Рік тому
If you are new to chromatin biology and epigenomics, you have come to the right place! In this part of our Chromatin Mapping Basics series, EpiCypher gives a basic introduction to chromatin structure and how it regulates gene expression. We explain the functions of histone post-translational modifications and other chromatin-associated proteins and how these features can be mapped using next-ge...
ChIP-seq review and analysis of chromatin mapping data
Переглядів 711Рік тому
Curious about chromatin mapping, but not sure where to start? In this part of our Chromatin Mapping Basics series we describe the general steps of chromatin mapping assays and data analysis, using chromatin immunoprecipitation sequencing (ChIP-seq) as our benchmark assay. We also review the advantages and disadvantages of ChIP-seq compared to more recent technologies, including CUT&RUN and CUT&...
CUT&RUN vs CUT&Tag: How they work and when to use each assay
Переглядів 5 тис.Рік тому
CUT&RUN and CUT&Tag assay are transforming epigenomics, allowing researchers to generate high resolution maps from small cell numbers with dramatic cost savings. But how do you know which assay to use? In this part of our Chromatin Mapping Basics series we review CUT&RUN and CUT&Tag chromatin mapping assay methods and how to select the right assay for your project. EpiCypher is the only company...
Analysis of chromatin mapping data: Sequencing tracks and heatmaps
Переглядів 850Рік тому
Chromatin mapping data analysis is complex, but forms an essential part of these experiments. In this short video we break down epigenomic sequencing analysis into basic steps, making the process easy to understand for scientists of all backgrounds. We also review the purpose of sequencing heatmaps and how to interpret these data. If you are interested in learning more about chromatin mapping a...
How mutations in PHIP could lead to neurodevelopment disorders and cancer
Переглядів 4203 роки тому
Morgan et al. investigated the functions of PHIP/BRWD2, a chromatin-associated protein linked to neurodevelopment disorders and many types of cancer. They confirmed PHIP binding to chromatin and show that PHIP helps regulate gene expression in cancer cells. The authors then used EpiCypher's dCypher chromatin binding platform to interrogate PHIP binding against defined nucleosome substrates, rev...
Leveraging Epigenomics to Accelerate Chromatin Research and Drug Discovery | AACR
Переглядів 6423 роки тому
EpiCypher's Director of Product Development, Andrea Johnstone, PhD, speaks at the American Association for Cancer Research's Exhibitor Spotlight Series. This brief talk covers EpiCypher's latest advances in the epigenomics field: CUTANA CUT&RUN technology, including the use of DNA-barcoded nucleosome spike-in controls for antibody validation and to monitor assay quality. Recorded September 9, 2...
EpiCypher: Advancing Epigenomics for Drug Development
Переглядів 1,1 тис.3 роки тому
EpiCypher is advancing the art of epigenomic profiling to decipher the chromatin code and accelerate drug development. These technologies will help usher in a new field of personalized medicine and improve human health.
Next Generation Genomic Mapping With CUTANA CUT&RUN | Epigenetic Therapeutic Targets Summit
Переглядів 2,1 тис.3 роки тому
EpiCypher CSO Michael-Christopher Keogh, PhD speaks at the 2021 Epigenetic Therapeutic Targets Summit. This brief talk covers the latest advances in CUTANA CUT&RUN technology, including the use of DNA-barcoded nucleosome spike-ins for antibody validation and to monitor assay quality. Recorded July 14, 2021. RESOURCES Learn more about CUTANA™ CUT&RUN Assays www.epicypher.com/technologies/cutana/...
EpiCypher | Leveraging quantitative epigenetics for drug development and clinical research
Переглядів 4803 роки тому
EpiCypher is pushing the boundaries of epigenetics research with innovative chromatin mapping tools, chromatin remodeling assays, defined nucleosome controls, and quantitative binding assays for target discovery. Learn more at EpiCypher.com
KAT8 displays complex-dependent histone acetyltransferase activity
Переглядів 1,3 тис.3 роки тому
Radzisheuskaya et al. demonstrate that the KAT8 acetyltransferase associates with two distinct protein complexes in vivo. Depending on the associated complex, KAT8 target different histone residues, with unique downstream impacts on the cell. This study is a fascinating example of the complexity of chromatin regulation, and how chromatin modifying enzymes can be modulated by protein interaction...
Decoding protein composition and function with Nuc-MS
Переглядів 2,3 тис.3 роки тому
Schechner et al. developed an improved mass spectrometry method to interrogate histone proteoforms. Unlike existing techniques, which use fragments of histones and thus lose the context of neighboring modifications, Nuc-MS isolates and analyzes intact histones. This method was developed using fully defined sets of modified recombinant designer nucleosomes from EpiCypher. See the published artic...
Histone H1 loss drives lymphoma by disrupting 3D chromatin architecture
Переглядів 8954 роки тому
Yusufova et al. discovered a novel set of oncomutations in the linker histone protein H1 that drive B cell lymphoma development. This mechanism was defined and validated using multiple techniques, including in vitro cell-based assays, mouse models, and EpiCypher's CUTANA CUT&RUN technology. See the published article here: www.nature.com/articles/s41586-020-3017-y
CUTANA™ CUT&Tag Technology: Ultra-sensitive Chromatin Profiling
Переглядів 16 тис.4 роки тому
EpiCypher CUTANA™ assays generate quality data with lower input and sequencing depth requirements. With CUT&Tag, go from cells to sequencing ready in a single tube! Learn more and order now at www.epicypher.com/CUT&Tag
CUT&RUN Protocol Walk-through | EpiCypher CUTANA™ CUT&RUN Assays
Переглядів 16 тис.4 роки тому
CUT&RUN Protocol Walk-through | EpiCypher CUTANA™ CUT&RUN Assays
Looking for an alternative to ChIP-seq? Try CUTANA™ ChIC / CUT&RUN sequencing!
Переглядів 2294 роки тому
Looking for an alternative to ChIP-seq? Try CUTANA™ ChIC / CUT&RUN sequencing!
ChIP-seq vs CUT&RUN : Learn the benefits of CUT&RUN sequencing for enhanced chromatin profiling
Переглядів 14 тис.4 роки тому
ChIP-seq vs CUT&RUN : Learn the benefits of CUT&RUN sequencing for enhanced chromatin profiling
Improved assay for quantifying citrullinated histones as markers of disease
Переглядів 1,1 тис.4 роки тому
Improved assay for quantifying citrullinated histones as markers of disease
An Introduction to SNAP-ChIP® Spike-in Controls
Переглядів 11 тис.4 роки тому
An Introduction to SNAP-ChIP® Spike-in Controls
Video Byte: H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape.
Переглядів 1,6 тис.5 років тому
Video Byte: H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape.
Molecular Cell video abstract: Examining the roles of H3K4 methylation states antibodies
Переглядів 6 тис.6 років тому
Molecular Cell video abstract: Examining the roles of H3K4 methylation states antibodies
One would also need to know what is in the columns of the heat-map.
excuse me, but viable cells should be more than 95% ? there is no way I can get these numbers after FACS and cryopreservation
good one
جامد جداااااااات😘👍
انا حبيبة شكرا لحضرتك
wonderful
So nice illustration, thanks. just one question; you applied ab treatment before permeabilization with digitonin in the protocol steps while in the illustration the opposite is mentioned
Great video!!
Can there be one for CUT&Tag please
We appreciate your feedback- and there will be a CUT&Tag video! We are still in the process of putting together all of our FAQs and recommendations for CUT&Tag. In the meantime, we do have a detailed CUT&Tag protocol available: www.epicypher.com/resources/protocols/cutana-cut-and-tag-protocol/
this method is AWESOME
Very valuable instruction